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Improved lipid breakdown

Improved lipid breakdown

Cite this article Nakayama, T. Journal lipis Biological Chemistry— Cardiovascular effects and safety of glucose-lowering drugs: current situation.

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Br J Nutr. Narasimhan A, Chinnaiyan M, Karundevi B. Ferulic acid regulates hepatic GLUT2 gene expression in high fat and fructose-induced type-2 diabetic adult male rat. Eur J Pharmacol. Maslowski KM, Vieira AT, Ng A, Kranich J, Sierro F, Yu D, et al. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR Samuel BS, Shaito A, Motoike T, Rey FE, Backhed F, Manchester JK, et al.

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A high-sugar and high-fat diet impairs cardiac systolic and diastolic function in mice. Int J Cardiol. Download references. This study was supported by National Natural Science Foundation of China and the National Key Research and Development Program of China YFD Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu, , Sichuan, China.

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, , Sichuan, China. Key Laboratory of Pig Industry Sciences, Rongchang, , Chongqing, China.

Chongqing Academy of Animal Sciences, Rongchang, , Chongqing, China. You can also search for this author in PubMed Google Scholar. conducted the animal work and the laboratory work, and wrote the manuscript.

and D. designed the experiment. and J. gave advice on the experiment design. analyzed the study data and wrote the manuscript. and H.

helped to revise the manuscript. All the authors have read and approved the final manuscript. Correspondence to Liangpeng Ge or Daiwen Chen. Experimental protocols and procedures used in the present experiment were approved by the Animal Care and Use Committee of Sichuan Agricultural University Chengdu, China under permit number DKY-B Ingredient composition of the milk powder as-fed basis.

Table S2. Ingredient composition of the basal diet as-fed basis. Table S3. Infusion volume of sterile saline or SCFAs mixture for each pig per day. Table S4.

Primer sequences used for real-time quantitative PCR. Open Access This article is licensed under a Creative Commons Attribution 4. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.

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Reprints and permissions. Zhou, H. et al. Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota. J Animal Sci Biotechnol 12 , 61 Download citation. Received : 02 November Accepted : 08 March Published : 06 May Anyone you share the following link with will be able to read this content:.

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Search all BMC articles Search. Download PDF. Abstract Background Previous studies have shown that exogenous short-chain fatty acids SCFAs introduction attenuated the body fat deposition in conventional mice and pigs. Methods Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators.

Conclusions Exogenous introduction of SCFAs might attenuate the fat deposition and to some extent improve the glucose control in the pig model, which occurred independently of the gut microbiota.

Materials and methods The experiment was conducted at the Experimental Swine Engineering Center of the Chongqing Academy of Animal Sciences CMA No. Animals Twelve neonatal GF pigs were delivered via hysterectomy from four multiparous Bama sows a native breed of China.

Experimental design and diet Among the six rearing isolators, three of them were designated as the FA group, and the other three isolators were treated as the GF group. Serum parameters measurement The concentrations of adiponectin, insulin, glucagon, glucagon-like peptide 1, and leptin in serum were detected by commercial enzyme-linked immunosorbent assay ELISA kits from Chenglin Co.

Determination of enzyme activity The frozen sample of the liver and longissimus dorsi approximately 1. Determination of protein levels The antibodies against the β-actin, GPR43, p-AMPK, AMPK, CPT-1B, and ACC were brought from Abcam Cambridge, MA, USA , Cell Signaling Technology Davers, MA , and Santa Cruz Biotechnology Inc.

Statistical analysis Data were analyzed in SAS 9. Results There were no differences in growth performance, nutrient digestibility, and relative organs weight between the GF and FA groups [ 33 ].

Serum parameters The impacts of oral administration of SCFAs on the serum parameters are shown in Table 1. Table 1 Effects of exogenously introduced SCFAs on the serum parameters in GF pigs a Full size table.

Table 2 Effects of exogenously introduced SCFAs on the activities associated with lipids metabolism in liver and longissimus dorsi of GF pigs a Full size table. Table 3 Effects of exogenously introduced SCFAs on the mRNA abundances for key factors associated with lipid metabolism in colon and liver of GF pigs a Full size table.

Table 4 Effects of exogenously introduced SCFAs on the mRNA abundances for key factors associated with lipid metabolism in longissimus dorsi of GF pigs a Full size table. Table 5 Effects of exogenously introduced SCFAs on the mRNA abundances for key factors associated with glucose metabolism in liver of GF pigs a Full size table.

Table 6 Effects of exogenously introduced SCFAs on the mRNA abundances for key factors associated with glucose metabolism in longissimus dorsi of GF pigs a Full size table.

Full size image. Table 7 Altered metabolites in the serum between FA and GF groups a Full size table. Discussion As is known to us, increasing dietary fiber intake contributes greatly to body weight and glucose tolerance [ 34 ]. Conclusions In summary, this study demonstrated that SCFAs may attenuate fat deposition and to some extent improve glucose control in the liver and longissimus dorsi, which occur independently of the gut microbiota.

Availability of data and materials The data were exhibited in the main manuscript and supplemental materials. References Delzenne NM, Cani PD, Everard A, Neyrinck AM, Bindels LB.

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Article CAS PubMed PubMed Central Google Scholar Carbone S, Canada JM, Buckley LF, Trankle CR, Billingsley HE, Dixon DL, et al. Acknowledgements Not applicable. Funding This study was supported by National Natural Science Foundation of China and the National Key Research and Development Program of China YFD View author publications.

Ethics declarations Ethics approval and consent to participate Experimental protocols and procedures used in the present experiment were approved by the Animal Care and Use Committee of Sichuan Agricultural University Chengdu, China under permit number DKY-B Consent for publication Not applicable.

Competing interests The authors declare that they have no conflict of interest. Supplementary Information. Additional file 1 : Table S1. Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.

About this article. Cite this article Zhou, H. Copy to clipboard. Journal of Animal Science and Biotechnology ISSN: Contact us Submission enquiries: Access here and click Contact Us General enquiries: info biomedcentral.

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PloS One 9 , e Download references. We thank the Kaneka Corporation of Japan for providing the mouse feed.

We also thank Drs. Kiyoshi Matsumoto and Takahiro Yoshizawa Research Center for Support to Advanced Science, Shinshu University for technical assistance and care of mice.

We thank Mr. Kiyokazu Kametani and Ms. Kayo Suzuki Research Center for Support to Advanced Science, Shinshu University for their skillful technical assistance. Department of Aging Biology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, , Japan.

Department of Advanced Medicine for Heath Promotion, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, , Japan. Department of Biological Sciences for Intractable Neurological Diseases, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, , Japan.

You can also search for this author in PubMed Google Scholar. conceived and designed experiments. and J. performed the experiments. analyzed the data. and H. contributed reagents and materials. wrote the paper. Correspondence to Zhe Xu. Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Open Access This article is licensed under a Creative Commons Attribution 4. Reprints and permissions. Xu, Z. Coenzyme Q10 Improves Lipid Metabolism and Ameliorates Obesity by Regulating CaMKII-Mediated PDE4 Inhibition. Sci Rep 7 , Download citation. Received : 04 May Accepted : 14 July Published : 15 August Anyone you share the following link with will be able to read this content:.

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Skip to main content Thank you for visiting nature. nature scientific reports articles article. Download PDF. Subjects Cell signalling Metabolic disorders. Abstract Our recent studies revealed that supplementation with the reduced form of coenzyme Q10 CoQ 10 H 2 inhibits oxidative stress and slows the process of aging in senescence-accelerated mice.

Introduction Imbalance between energy input and output can lead to the accumulation of excess fat, causing obesity. Results CoQ 10 H 2 inhibited weight gain and improved metabolic syndrome in KKAy mice In this study, dietary supplementation with CoQ 10 H 2 was employed to investigate the effect of CoQ 10 H 2 on metabolic syndrome in KKAy mice.

Figure 1. Full size image. Figure 2. Figure 3. Figure 4. Figure 5. Discussion It is well known that CoQ 10 H 2 is a powerful antioxidant that can potently inhibit the generation of oxygen free radicals and oxidative stress damage, thereby ameliorating age-associated disease.

Figure 6. Methods Animals 7-week-old female KKAy mice were purchased from CLEA Japan Inc. Cell Culture The human hepatoma HepG2 cell line was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan, and 3T3L1 cells were purchased from the Japanese Cancer Research Resources Bank.

Western blotting and immunoprecipitation Tissues and cells were lysed in cell lysis buffer Cell Signaling Technology, MA supplemented with protease inhibitors Sigma Aldrich, MO.

Real-time RT-PCR Total RNA was extracted using TRIzol Reagent Invitrogen, CA , followed by treatment with DNA-Free Applied Biosystems, CA to remove contaminating DNA and then subjected to reverse transcription using an Omniscript RT kit Applied Biosystems, CA with random primers Applied Biosystems, CA.

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The gut lipis is a central lipiv of Body composition measurement system metabolism. Improved lipid breakdown composition and function of the gut microbiota is dynamic and Imoroved by diet properties such as Improved lipid breakdown amount and composition of lipids. Hence, dietary lipids breakvown influence host physiology through interaction with the gut microbiota. Lipids affect the gut microbiota both as substrates for bacterial metabolic processes, and by inhibiting bacterial growth by toxic influence. The gut microbiota has been shown to affect lipid metabolism and lipid levels in blood and tissues, both in mice and humans. Furthermore, diseases linked to dyslipidemia, such as non-alcoholic liver disease and atherosclerosis, are associated with changes in gut microbiota profile. Improved lipid breakdown Journal breajdown Animal Lipiid and Brekdown volume 12Improved lipid breakdown number: 61 Improved lipid breakdown mIproved article. Metrics Improved lipid breakdown. Previous studies Nutrient-rich meals shown that exogenous short-chain fatty acids SCFAs introduction attenuated the body fat deposition in conventional mice and pigs. However, limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut microbiota. This study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free GF pig model. Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators. All pigs were hand-fed with sterile milk powder for 21 d, then the sterile feed was introduced to pigs for another 21 d.

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